DNA flow cytometric analysis in variable types of hydropic placentas

DNA flow cytometric analysis in variable types of hydropic placentas

Blog Article

Background: Differential Ringette - Equipment diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management.Objective: We analyzed the nuclear DNA content of various types of hydropic placentas by flowcytometry.Materials and Methods: DNA ploidy analysis was performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (complete and partial moles), formalin-fixed, paraffin-embedded tissue samples by flow cytometry.The criteria for selection were based on the histopathologic diagnosis.Results: Of 10 cases histologically diagnosed as complete hydatiform mole, 9 cases yielded diploid histograms, and 1 case was tetraploid.

Of 10 partial hydatidiform moles, 8 were triploid and 2 were diploid.All of 20 cases diagnosed as spontaneous abortions (hydropic and Evaporative Cooler Pump non-hydropic) yielded diploid histograms.Conclusion: These findings signify the importance of the combined use of conventional histology and ploidy analysis in the differential diagnosis of complete hydatidiform mole, partial hydatidiform mole and hydropic abortion.